RecA

نویسندگان

  • Roberto Galletto
  • Stephen C. Kowalczykowski
چکیده

then most lines thereafter crossed out. Madame Auclair, the French violinist, had a gentler approach. Out of central casting, as they say: dark glasses, cigarette dangling, hoarse, accented voice. A friend went for a special violin lesson, and asked whether he might tape record this important event in his life. “Of course, my boy.” He played a bit and she said: “Very nice. There are some good things about your playing, very good. Now turn off the tape recorder.” When I am struggling over yet another of my obscurely written drafts I sometimes recall: amateurs play music ‘in general’; professionals play each note. And so I present to a tough-minded friend one paragraph — just one — and when that is reported to be transparent I go on to the rest. But even if I have followed the rules I mentioned above, and even if that first paragraph seemed fine at the time, now, in view of what else I have written, that first paragraph might have to go, or be seriously recast. Each paragraph is an experiment — you might not know for some time whether it is any good. There is a theme here, beautifully expressed by a friend who was going through the agonies of the “just the first paragraph” method in attempting to re-write a book. I hadn’t heard from him in a while and began to worry — had I been too tough? — and he wrote: “The only reason I hadn’t sent it (the new paragraph) already is that I didn’t want to disappoint you. But I realize that the only way you can help me is if I continue to disappoint you. So here it is...” All my teachers, whatever their methods, were trying to help me, and I love them for it. Heifetz I wouldn’t be so sure about. Rules are one thing, but in the end communication is all: at the end of a pleasant interview with a fine scientist of foreign extraction she shook my hand and said: “Its been a pleasure talking to me.”

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

بیان ژن recA درموتانdinI- مقاوم به سیپروفلوکسازین اشریشیا کلی

مقدمه: در پاسخ به توسعه داروها، ریزموجودات با سازوکارهای متنوعی به آنها مقاوم می‌شوند. در سال‌های اخیر، شیوع مقاومت به فلوروکینولون‌ها مانند سیپروفلوکساسین در اشریشیا کلی به‌طور چشمگیری افزایش یافته است. موتان‌زایی ناشی از SOS، مقاومت به فلوروکینولون‌ها را القا می‌کند. ژن dinI، عضو تنظیمی پاسخ SOS است و پروتئین DinI را کد می‌کند که تعدیل‌کننده مثبت و منفی عملکرد RecA است. در بررسی‌های پیشین مشخ...

متن کامل

Determining Difference in Evolutionary Variation of Bacterial RecA proteins vs 16SrRNA Genes by using 16s_Toxonomy Tree

Background and Aims: The rate of variation in various genes of a bacterial species is different during evolution. Therefore, in systematic bacterial studies many researchers compare the phylogenetic tree of a particular gene to the standard tree of an rRNA gene. Regarding the importance of 16SrRNA gene and the evolutional process of RecA protein family, we investigated the changes in the select...

متن کامل

Regulation of bacterial RecA protein function.

The RecA protein is a recombinase functioning in recombinational DNA repair in bacteria. RecA is regulated at many levels. The expression of the recA gene is regulated within the SOS response. The activity of the RecA protein itself is autoregulated by its own C-terminus. RecA is also regulated by the action of other proteins. To date, these include the RecF, RecO, RecR, DinI, RecX, RdgC, PsiB,...

متن کامل

RecA K72R filament formation defects reveal an oligomeric RecA species involved in filament extension.

Using an ensemble approach, we demonstrate that an oligomeric RecA species is required for the extension phase of RecA filament formation. The RecA K72R mutant protein can bind but not hydrolyze ATP or dATP. When mixed with other RecA variants, RecA K72R causes a drop in the rate of ATP hydrolysis and has been used to study disassembly of hydrolysis-proficient RecA protein filaments. RecA K72R ...

متن کامل

Purification and Characterization of the RecA Protein from Neisseria gonorrhoeae

The strict human pathogen Neisseria gonorrhoeae is the only causative agent of the sexually transmitted infection gonorrhea. The recA gene from N. gonorrhoeae is essential for DNA repair, natural DNA transformation, and pilin antigenic variation, all processes that are important for the pathogenesis and persistence of N. gonorrhoeae in the human population. To understand the biochemical feature...

متن کامل

Transcriptional and mutational analyses of the Streptomyces lividans recX gene and its interference with RecA activity.

The role of the 20,922-Da RecX protein and its interference with RecA activity were analyzed in Streptomyces lividans. The recX gene is located 220 bp downstream of recA. Transcriptional analysis by reverse transcriptase PCR demonstrated that recX and recA constitute an operon. While recA was transcribed at a basal level even under noninducing conditions, a recA-recX cotranscript was only detec...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Current Biology

دوره 17  شماره 

صفحات  -

تاریخ انتشار 2007